The Mouse DNA Topoisomerase 2-alpha ELISA Kit is a specialized assay designed to quantitatively measure DNA topoisomerase 2-alpha (TOP2A) levels in biological samples. As a critical enzyme involved in DNA replication, transcription, and chromosomal segregation, TOP2A plays a vital role in cell proliferation and genome stability. This ELISA kit provides researchers with a sensitive, reliable method to study TOP2A expression, making it invaluable for cancer research, drug development, and molecular biology studies.
Importance of DNA Topoisomerase 2-alpha
DNA topoisomerase 2-alpha is a type II topoisomerase essential for resolving DNA supercoiling and entanglements during replication and transcription. It is particularly significant in rapidly dividing cells, where its inhibition has been a target for chemotherapy drugs such as etoposide and doxorubicin. Learn more about the function and structure of TOP2A at NIH and PubMed.
Features of the Mouse DNA Topoisomerase 2-alpha ELISA Kit
- High Sensitivity and Specificity: Detects low levels of TOP2A with minimal cross-reactivity.
- Quantitative Measurement: Provides precise data for expression analysis.
- Versatile Applications: Suitable for serum, plasma, and tissue homogenates.
- User-Friendly Protocol: Includes pre-coated plates, buffers, and reagents for straightforward use.
For detailed product specifications, visit CDC Laboratory Resources or FDA.
Applications in Research
The Mouse DNA Topoisomerase 2-alpha ELISA Kit is widely used in:
- Cancer Research: Investigating TOP2A expression as a biomarker for cancer progression and therapeutic response. Explore related studies at Cancer.gov.
- Drug Screening: Assessing the efficacy of topoisomerase inhibitors in preclinical trials. For more on drug screening, see NCBI Drug Discovery Resources.
- Cell Cycle Studies: Analyzing the regulation of TOP2A during cell division. For protocols on cell cycle analysis, refer to NIH’s Molecular Biology Resources.
How the ELISA Works
This ELISA kit employs a sandwich assay format. A monoclonal antibody specific to TOP2A is pre-coated on the plate. After adding the sample, the bound TOP2A is detected using a biotin-conjugated antibody, followed by a streptavidin-HRP conjugate. The enzymatic reaction produces a measurable colorimetric signal proportional to the TOP2A concentration.
For step-by-step assay guidance, consult the USDA Laboratory Methods.
Related Research
- Role of TOP2A in Cancer: Studies have shown that TOP2A overexpression is linked to aggressive tumor phenotypes and poor patient outcomes. Access relevant literature at PubMed.
- Targeting TOP2A in Therapy: Research continues to focus on developing more selective inhibitors for TOP2A. Learn more about therapeutic strategies from NIH’s Cancer Institute.
Storage and Handling
Proper storage ensures optimal kit performance:
- Short-Term Storage: Store at 2–8°C.
- Long-Term Storage: Avoid repeated freeze-thaw cycles; aliquot and freeze components at -20°C.
Find detailed storage recommendations at the CDC Laboratory Guidelines.
Regulatory Compliance
The Mouse DNA Topoisomerase 2-alpha ELISA Kit meets international research standards, ensuring high-quality and reproducible results. For regulatory guidelines, visit FDA and WHO Laboratory Standards.
Additional Resources
Conclusion
The Mouse DNA Topoisomerase 2-alpha ELISA Kit is a versatile and reliable tool for studying TOP2A, providing valuable insights into cellular processes and disease mechanisms. Whether used for cancer research, drug development, or basic biology, this kit is a cornerstone for advancing scientific discovery. For additional details or purchasing inquiries, visit trusted resources like NIH, FDA, and CDC.
For further technical support or product information, reach out to suppliers listed at FDA Approved Diagnostic Kits or the NIH Antibody Resource.