High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions
We describe a streamlined and systematic technique for cloning inexperienced fluorescent protein (GFP)-open studying body (ORF) fusions and assessing their subcellular localization in Arabidopsis thaliana cells. The sequencing of the Arabidopsis genome has made it possible to undertake genome-based approaches to find out the perform of every protein and outline its subcellular localization. This is …